1.
Production Of Synbiotic Product Containing Galacto-Oligosaccharides And Saccharomyces Boulardii And Evaluation Of Its In Vitro Bifidogenic Effect
by Farah Javed (2013-Mphil-1110) | Dr. Sanaullah Iqbal | Dr. Zubair Farooq | Miss Faiza Masood.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Food are not only used to satisfy hunger or provide nutritional support but also used to prevent diseases that are based on nutrition and thus improve the health of consumers (Menrad, 2003).A food that is same in appearance and consumption to conventional food but has physiological benefits and prevents diseases is known as functional food (Sangwan et al. 2011). Functional foods are used to provide benefit to intestinal microflora. The most important functional food are prebiotic and probiotic. The combination of prebiotic and probiotic are known as synbiotics. The symbiotic is effective as that of prebiotic and probiotic. The probiotic is used to reduce the impurities from prebiotic mixture. This is very important to know the functional property of functional food (Sangwan et al. 2014) that is galactooligosaccharides (GOS) in present research.
GOS are most effective prebiotic which are beneficial to intestinal microbiota. Other health effects are stool improvement, mineral absorption, weight management and also improve the growth of Bifidobacterium and Lactobacillus (Macfarlane et al. 2008). The Saccharomyces boulardii is a non-pathogenic yeast and used as probiotic. It is thermo tolerant yeast and optimally grows at body temperature i.e; 37ºC. Due to this property S. boulardii can be used for the treatment of gastrointestinal diseases. Another function of this yeast is the reduction of monosaccharides from prebiotic mixture.
The purposes of present research work are to reduce the monosaccharides form mixture of GOS and the production of synbiotic product containing GOS and S. boulardii. For this GOS
were produced through transgalactosylation procedure using lactose as substrate. S. boulardii was grown separately and incubated with the mixture of GOS. After that symbiotic product was formed and lyophilized it. The total count of yeast was determined in lyophilized mixture. The in vitro bifidogenic effect of GOS and lyophilized product was also checked.
Availability: Items available for loan: UVAS Library [Call number: 2291-T] (1).
2.
Effect Of Omega-3 Enriched Eggs On Oxidative Stress In Healthy Human Subjects
by Mubeen Rafiq (2008-VA-432) | Dr. Muhammad Nasir | Prof. Dr. Nosheen Aziz | Dr. Zubair Farooq | Dr. M. Yasin Tipu.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Oxidative stress caused by increased production of free radicals due to altered antioxidant enzyme defense, increased lipid peroxidation and reduced levels of essential fatty acids. It is possible that the oxidative injury can be prevented by dietary supplementation of omega 3 fatty acid and antioxidants.
Omega-3 fortified eggs might be helpful in decreasing oxidative stress in healthy human subjects. Efficacy study of omega-3 fortified eggs was conducted against oxidative stress through biochemical analysis of biomarkers of oxidative stress e.g., catalase and superoxide dismutase.Eighteen volunteers were selected and divided in three groups (control, omega-3 supplements + vitamin E and omega-3 fortified eggs). At 0, 30th and 60th day, blood sample was collected and further examined.
The data will be analyzed through Analysis of Variance with repeated measures (rANOVA) technique. The significance level will be defined as P ≤0.05 and means will be compared through Duncan’s Multiple Range test for significance difference.
Omega-3 bio fortified eggs that would be useful for decreasing the oxidative stress in healthy human subjects.P value is less than 0.05 and results indicate that omega 3 fortified eggs may helpful in decreasing oxidative stress in humans. Also results showed that omega 3 fortified eggs are helpful in improving lipid profile by increasing HDL levels and decreasing LDL levels, decrease in triglyceride and cholesterol also a plus point of this product as it is economic, easy to access and nutritious.
Availability: Items available for loan: UVAS Library [Call number: 2358-T] (1).
3.
Assessment Of Variour Nutrient's Intake In Pregnant And Lactating Women Through Food Frequency Checklist And 24-Hour Dietary Recall In Lahore
by Hasiba Munir (2008-VA-435) | Dr. Muhammad Nasir | Prof. Dr. Nosheen Aziz | Dr. Zubair Farooq | Dr. Yasin Tipu.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Poor nutritional status during pregnancy is a contributory factor in maternal and infant morbidity and mortality. Widespread household food insecurity (58.1% households are food insecure) and poor knowledge about nutrient requirements in pregnant and lactating women are the major cause of nutritional deficiencies. This study is planned to assess nutrients’ intake among pregnant and lactating women and to compare with standard requirements.
A cross sectional survey design was used to assess the nutrients’ intake of 110 pregnant and 110 lactating women from 6 Union Councils (two urban, two per-urban and two rural Union Councils) of Lahore and Rajanpur districts. Nutrients’ intake was assessed by using a pre-tested structured questionnaire to interview the pregnant and lactating women at one point in time. Weight, height and mid upper arm circumference was measured through anthropometric measures, 24 hour dietary recall, and food frequency was used to assess nutrients’ intake.
The data was analyzed bychi-square and cross tabs using SPSS version 20.0 and was presented in percentage, frequencies and charts to assess the nutritional deficiencies.Significance level was defined as p≤ 0.05.
The results shows that majority of the women were deficient in energy and nutrient's intake through daily diet. In both districts pregnant and lactating women were not consuming their required amount of various nutrient's according to their maternal status. Results show significant difference in Lahore and Rajanpur districts most of the nutrient's deficiency was present in Rajanpur as compare to Lahore. Severely low dietary intake of various nutrients was calculated and analyzed. According to the results dietary intake of various nutrients was severely low in selected women with following percentage protein was 39.1%, folate 40%, B12 20.5%, B6 74.5%, vitamin C 30.5%, vitamin A 14.1%, vitamin D 69.1%, calcium 26.8%, iron 96.8% and zinc 70.9%. These results shows very adverse situation of important nutrient's intake of pregnant and lactating women through diet.
Availability: Items available for loan: UVAS Library [Call number: 2357-T] (1).
4.
Evaluation Of Lipid Lowering Effect Of Terminalia Chebula In Hyperlipidemic Patients
by Abdur Rehman (2013-VA-780) | Dr. Muhammad Nasir | Dr. Zubair Farooq | Dr. Aqeel Javeed.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Hyperlipidemia is regarded as leading cause of cardiovascular diseases and atherosclerosis and found to be best recognized modifiable factor for heart diseases and atherosclerosis. The present study constitutes on T. chebulahaving hypolipidemic activity. Sixty patients were studied in four groups and treated with T.chebula (150 mg hydroalcholicextract) for group 1 (15 patients), T.chebula(150mg hydroalcholic extract) + Atorvastatin (10 mg) for group 2 (15 patients), T. chebula (75 mg hydroalcholic extract) + Atorvastatin (5 mg) for group 3 and Atorvastatin (10 mg) for group 4 respectively for 12 weeks. The primary endpoints were mean changes in LDL-C, VLDL-C, HDL-C, Triglycerides and Total Cholesterol levels at 0, 30, 60 and 90 days. Data are expressed as means ± standard error of mean (SEM) and minimum – maximum and was analyzed using Descriptive Statistics and Repetitive Measures. Values of p<0.05 were considered statistically significant.The hydroalcoholic extract of T. chebula exhibits significant hypolipidemic activity when applied in different doses as compared to statin group. Lipid parameters like Cholesterol, Triglycerides, LDL and VLDL were decreased in all the 4 designed groups. However the statin groups have relatively high potential of decreasing Cholesterol, Triglycerides, LDL and VLDL when compared with the results of T. chebula extract. Group 3 have least hypolipidemic activity. Group 2 receiving exhibits highest results when compared with all the other 3 groups. Group 1 has the ability to significantly decrease Cholesterol, Triglycerides, LDL and VLDL when compared with group 4. Group 4 was taken as standard and found to significantly reduce the all lipid parameters used during the study.These results suggests a pharmacological indication on the traditional uses of T. chebula pericarp for hyperlipidemia and it can be determined that T. chebulahave a strong ability to reduce high lipid profile. The findings of proposed research are helpful in reducing modifiable risk factor for cardiovascular diseases. Availability: Items available for loan: UVAS Library [Call number: 2716-T] (1).
5.
Assessment Of Glycemic Modulation In Diabetic Sprague Dawley Rats Through Feeding Camel Milk Whey
by Rabia Akram (2014-VA-769) | Dr. Muhammad Nasir | Prof. Dr. Habib-ur-Rehman | Dr. Zubair Farooq.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The number of people diagnosed with diabetes is increasing tremendously exhausting the ability of health care systems to deal with the epidemic. Therefore it is necessary to find scientific merits in traditional folk remedies to justify their claims. Number of investigational studies on the traditional belief that regular consumption of camel milk helps in the prevention and control of diabetes are done by experimentations on different animal models have given significant results. Therefore present project was designed for evaluation of camel milk whey as functional food against diabetes mellitus. In the first part of study whey was extracted and in the second part of study efficacy of camel milk whey as functional food against diabetes mellitus was evaluated in male Sprague dawley rats. Seventy two rats were induced diabetes by injecting Streptozotocin (50 mg/ kg) dissolved in cold 10mM of citrate buffer at pH of 4.5. Diabetes was induced in group I, II and III rats and feed on three types of diet (standard diet + water, standard diet + raw camel milk and standard diet + camel milk whey) respectively. After the induction of diabetes it was confirmed by checking the 8 hour fastening blood glucose level using the Abbot glucometer, rats with blood glucose greater than 200mg/dl were considered diabetic. Base line reading were recorded by decapitating 6 rats from each group. Parameters which were consider included serum blood glucose level(mg/dl), TGL (mg/dl), cholesterol (mg/dl), HDL (mg/dl) and LDL (mg/dl) further more to observe the changes at cellular level histopathology of liver and pancreas was also performed. Therefore the present study consisting of duration of 6 weeks showed that camel milk whey is concentrated with number of beneficial proteins that are helpful in reducing the blood glucose level significantly in diabetic rats as well as it also remarkably in creased the level of LDL. The histopathology report of organs i.e. liver and pancreas showed improvement gradually with the ongoing treatment and these improvements strongly prove
Summary
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that composition of serum not only improved temporarily but long term use of camel milk whey could not only help to improve serum composition but it also cases regeneration of degraded cells of pancreas and also improved the sinusoidal congestion in liver. Availability: Items available for loan: UVAS Library [Call number: 2773-T] (1).
